|
KMID : 0352719990230010055
|
|
Journal of Ginseng Research
1999 Volume.23 No. 1 p.55 ~ p.59
|
|
|
A Simple Mehtod for Conjugation Between Ginsenoside Rf and Bovine Serum Albumin
|
|
Seung Yeol Nah/1So Rah Yoon
1JIn Ju Nah/1Seok Choi/1Chang Ho Son/1Dong Ho Shin/1Seung Yeol Nah/2Sung Ryong Ko
|
|
|
Abstract
|
|
|
|
For the first time we have developed a simple method for conjugation between ginsenoside Rf (Rf) and bovine serum albumin (BSA) or ovalbumin (OVA) by a periodate oxidation method. The Rf-BSA conjugate is confirmed by ultraviolate (UV) scanning, thin layer chromatography (TLC), and SDS-gel electrophoresis. In UV scanning Rf showed three small peaks approximately at 230, 265 and 280 nm. BSA showed a peak at 280 nm. The Rf-BSA conjugate showed right shifted three peaks that BSA alone did not show. In TLC analysis, the Rf-BSA conjugate did not show mobility in silica gel but showed a slight stream of trace. In SDS-gel electrophoresis, Rf-BSA conjugate show a slight less mobility than BSA alone. Rf-OVA conjugate also showed similar patterns with Rf-BSA conjugate. These results demonstrated that periodate oxidation method could be used to produce a stable Rf-BSA or Rf-OV A conjugate and also could be applied for other ginsenoside(s).
|
|
|
KEYWORD
|
|
|
Ginsenoside Rf, BSA, Periodate oxidation, Rf-BSA conjugate,
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|